TILLinG

TILLinG integrates Targeting Induced Local Lesions in Genomes (TILLinG) with Illumina next-generation sequencing to detect and annotate induced mutations in tetraploid wheat exomes for mutation discovery, functional annotation, and selection.

Key Features:

• Mutation Discovery and Selection: Enables creation and analysis of large populations with thousands of mutations to identify and select induced variants for gene functional analysis and breeding.
• Advanced Sequencing Integration: Utilizes short-read Illumina sequencing (2x125 bp) with an exome capture platform to generate exome data from durum wheat lines.
• Optimized Reference Sequence: Aligns reads to a 484.4 Mbp consolidated exome reference derived from durum wheat cultivars Svevo and Kronos to improve alignment and variant calling for lines derived from UAD0951096_F2:5.
• Searchable Database: Stores variant calls and supports prediction of zygosity and extraction of flanking sequences for marker development.
• Bioinformatics Pipeline and Functional Annotation: Implements variant calling and functional annotation of the durum wheat genome to facilitate rapid identification of mutations for varietal improvement.

Scientific Applications:

• Functional Genomics: Supports gene function studies in tetraploid wheat through precise mutation discovery and annotation.
• Breeding Programs: Provides induced genetic diversity and marker-ready flanking sequences to support selection in breeding initiatives.
• Genomic Research: Enables exome-level analyses of durum wheat genetics, contributing to variant discovery and genome annotation across mutant collections.

Methodology:

Illumina 2x125 bp exome-capture reads are aligned to the 484.4 Mbp Svevo–Kronos consolidated exome reference, followed by variant calling, functional annotation, storage in a searchable database, zygosity prediction, and extraction of flanking sequences for marker development.