TM3 seq

TM3 seq performs 3'-enriched RNA-seq library preparation using Tn5 transposase-mediated tagmentation to enable cost-effective, high-throughput transcriptome profiling while preserving individual sample identity.

Key Features:

• 3'-enriched library preparation: Uses a 3'-enrichment strategy to target transcript 3' ends for transcriptome coverage.
• Tn5 transposase-mediated tagmentation: Employs Tn5 transposase to fragment RNA and insert sequencing adapters in a single step.
• High throughput: Can prepare libraries for up to 96 samples within a six-hour period with approximately three hours of hands-on time.
• Cost efficiency: Reduces reagent cost to an estimated $1.5 per sample compared to commercial kits.
• Sample integrity preservation: Preserves the identity of each sample throughout library preparation to support individual library generation and re-sequencing.
• Quality and reliability: Validated on human and Drosophila melanogaster RNA samples with transcriptome recovery comparable to the NEBNext kit.

Scientific Applications:

• Large-scale RNA-seq experiments: Suitable for studies where throughput and per-sample cost are limiting factors.
• Quantitative genetics: Enables expression profiling across large sample sets for quantitative genetics analyses.
• Medical genomics: Applicable to genome-wide expression studies in medical genomics.
• Developmental biology: Supports transcriptome profiling in developmental biology research.
• Ecology: Facilitates collection of genome-wide expression data in ecological studies.
• Individual-sample studies and re-sequencing: Appropriate for projects that require preservation of sample identity for downstream analyses or re-sequencing.

Methodology:

Tn5 transposase-mediated tagmentation fragments RNA and adds sequencing adapters in a single step, and the protocol employs a 3'-enrichment strategy.