Thesaurus

Thesaurus identifies and quantifies phosphopeptide positional isomers from parallel reaction monitoring (PRM) and data-independent acquisition (DIA) mass spectrometry data to resolve site-specific phosphorylation in signaling networks.

Key Features:

• Detection and Quantification: Identifies and measures phosphopeptide positional isomers using site-specific fragment ions derived from PRM and DIA mass spectrometry.
• Versatility Across PTMs: Handles phosphorylation and additional post-translational modifications (PTMs) for broad proteomic analyses.
• Application in Complex Pathways: Has been applied to analyze phosphorylation events in the PI3K/AKT signaling pathway, distinguishing regulatory differences at neighboring phosphorylation sites.

Scientific Applications:

• Protein Regulation Studies: Discriminates positional isomers to reveal site-specific regulatory mechanisms of protein function.
• Signaling Pathway Analysis: Enables analysis of phosphorylation dynamics within signaling networks such as PI3K/AKT.
• Disease and Therapeutic Research: Supports studies of disease-related signaling pathways and can inform therapeutic development by resolving site-specific phosphorylation patterns.

Methodology:

Analyzes site-specific fragment ions generated in PRM and DIA mass spectrometry experiments using computational algorithms that integrate mass spectrometry data to detect and quantify positional isomers.