Vireo

Vireo is a cutting-edge computational tool to demultiplex single-cell RNA-seq data from pooled experimental designs, a method that increases throughput and addresses batch variation in the analysis of multiple samples. Traditional demultiplexing strategies have depended on the availability of complete genotype data from the samples within the pool, a requirement that constrains their utility, especially when genetic variation is not the focus of the study.

Vireo distinguishes itself by offering a solution that does not require comprehensive genotype information, making it particularly valuable when only partial or no genotype data is available. It achieves this through a computationally efficient Bayesian model capable of accurately reconstructing the sample identity of each cell based on genetic variants that naturally segregate between samples in the pool, serving as a natural barcode for cell demultiplexing.