VisioProt-MS

VisioProt-MS visualizes deconvoluted intact protein mass spectrometry (MS) data as interactive 2D LC-MS maps and 3D LC-MS plots to analyze proteoforms using deconvoluted molecular weights, retention times, and signal intensity.

Key Features:

• Compatibility: Compatible with algorithms and software for integrating and deconvoluting top-down and intact protein MS data.
• Interactive mapping: Generates interactive 2D maps and 3D LC-MS plots representing deconvoluted molecular weights, retention times, and MS signal intensity.
• MS/MS representation: Provides graphical representations tailored for MS and tandem mass spectrometry (MS/MS) analysis of proteoforms in complex samples.
• Acquisition and search optimization: Aids optimization of top-down acquisition and search parameters through visualization of LC-MS maps.

Scientific Applications:

• Proteoform Analysis: Examination of proteoforms via deconvoluted molecular weights and retention times.
• Parameter Optimization: Optimization of top-down MS acquisition and search parameters based on visual inspection of LC-MS data.
• Complex Sample Analysis: Analysis of complex biological samples, exemplified by the human proteasomal 20S core particle.

Methodology:

Plots deconvoluted molecular weights against retention times and signal intensity to produce interactive 2D maps and LC-MS visualizations.