WormBook

WormBook provides a comprehensive, peer-reviewed compendium of biological knowledge and experimental protocols for Caenorhabditis elegans and related nematodes.

Key Features:

• WormMethods: Detailed protocols and experimental procedures specific to C. elegans and nematode research.
• WormHistory: Personal accounts and historical perspectives from pioneers in C. elegans research.
• Worm Breeder's Gazette: A biannual newsletter facilitating exchange of methods and observations among nematode researchers.
• Additional Chapters: Peer-reviewed chapters available through GENETICS that extend coverage beyond core sections.

Scientific Applications:

• Neuronal development and function: Support studies of neuronal development and function using the anatomically defined C. elegans nervous system.
• Genetic and developmental studies: Enable genetic and developmental investigations leveraging C. elegans translucence, short life cycle, and extensive genetic tools.
• Protein expression and localization: Facilitate investigation of protein expression and distribution using transgenic markers such as green fluorescent proteins, noting potential discrepancies with endogenous protein levels.
• Antibody-based validation: Support antibody-based validation and immunohistochemistry to examine endogenous protein localization and developmental changes.

Methodology:

Provides an immunostaining protocol tailored to C. elegans and discusses immunohistochemistry, antibody generation challenges, and approaches to access the nervous system through the cuticle across developmental stages.