YeastSpotter
YeastSpotter segments yeast microscopy images into individual cells to provide reproducible preprocessing for quantitative image analysis.
Key Features:
- Parameter-free segmentation: Performs segmentation without manual parameter tuning to standardize results across datasets.
- Generalizability: Applicable to a wide range of yeast microscopy images.
- Accurate cell delineation: Distinguishes individual yeast cells with high accuracy for downstream analyses.
- Automation: Automates the segmentation process using advanced image processing algorithms.
Scientific Applications:
- Quantitative cell biology: Enables precise cell identification required for quantitative cell biology studies.
- Phenotypic screening: Facilitates identification of individual cells in phenotypic screening experiments.
- High-throughput imaging experiments: Serves as preprocessing for large-scale imaging datasets.
- Morphological studies: Supports morphological analysis by delineating cell boundaries.
- Single-cell assays: Provides per-cell segmentation necessary for single-cell assays.
Methodology:
Employs advanced image processing algorithms to automate segmentation and distinguish individual yeast cells.
Topics
Details
- License:
- MIT
- Maturity:
- Mature
- Cost:
- Free of charge
- Tool Type:
- web application
- Operating Systems:
- Linux, Windows, Mac
- Programming Languages:
- Python
- Added:
- 8/9/2019
- Last Updated:
- 11/24/2024
Operations
Publications
Lu AX, Zarin T, Hsu IS, Moses AM. YeastSpotter: accurate and parameter-free web segmentation for microscopy images of yeast cells. Bioinformatics. 2019;35(21):4525-4527. doi:10.1093/bioinformatics/btz402. PMID:31095270. PMCID:PMC6821424.