YeastSpotter

YeastSpotter segments yeast microscopy images into individual cells to provide reproducible preprocessing for quantitative image analysis.


Key Features:

  • Parameter-free segmentation: Performs segmentation without manual parameter tuning to standardize results across datasets.
  • Generalizability: Applicable to a wide range of yeast microscopy images.
  • Accurate cell delineation: Distinguishes individual yeast cells with high accuracy for downstream analyses.
  • Automation: Automates the segmentation process using advanced image processing algorithms.

Scientific Applications:

  • Quantitative cell biology: Enables precise cell identification required for quantitative cell biology studies.
  • Phenotypic screening: Facilitates identification of individual cells in phenotypic screening experiments.
  • High-throughput imaging experiments: Serves as preprocessing for large-scale imaging datasets.
  • Morphological studies: Supports morphological analysis by delineating cell boundaries.
  • Single-cell assays: Provides per-cell segmentation necessary for single-cell assays.

Methodology:

Employs advanced image processing algorithms to automate segmentation and distinguish individual yeast cells.

Topics

Details

License:
MIT
Maturity:
Mature
Cost:
Free of charge
Tool Type:
web application
Operating Systems:
Linux, Windows, Mac
Programming Languages:
Python
Added:
8/9/2019
Last Updated:
11/24/2024

Operations

Publications

Lu AX, Zarin T, Hsu IS, Moses AM. YeastSpotter: accurate and parameter-free web segmentation for microscopy images of yeast cells. Bioinformatics. 2019;35(21):4525-4527. doi:10.1093/bioinformatics/btz402. PMID:31095270. PMCID:PMC6821424.

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