A3D Database

A3D Database provides structure-based predictions of aggregation propensity for over 17,000 non-membrane human proteins by analyzing AlphaFold models with Aggrescan3D.

Key Features:

• Structure-Based Predictions: Uses Aggrescan3D to compute aggregation propensity on protein structures predicted by AlphaFold.
• Comprehensive Dataset: Contains predictions for over 17,000 non-membrane human proteins.
• Graphical Visualization: Provides graphical visualization of aggregation propensities and structural features.
• Mutation Analysis: Evaluates effects of specific mutations on protein solubility and stability.

Scientific Applications:

• Protein Misfolding Disease Research: Maps aggregation-prone regions in human proteins to support studies of protein misfolding diseases.
• Therapeutic Protein Design: Guides design of more soluble therapeutic proteins by identifying aggregation-prone regions and mutation effects.
• Genetic Variant Impact Assessment: Facilitates assessment of how genetic variants may influence protein aggregation, solubility, or stability.

Methodology:

Computationally integrates AlphaFold structural predictions with Aggrescan3D aggregation analyses to assess aggregation propensities across the human proteome.