Ab-Ligity

Ab-Ligity identifies sequence-dissimilar antibodies that target the same epitope using structure-based analysis of antibody-antigen interfaces.

Key Features:

• Structure-Based Similarity Measure: Employs a structure-focused approach to compare antibody-antigen interfaces rather than relying on sequence similarity.
• Predicted Paratopes Utilization: Leverages predicted paratopes from model antibody structures to assess and identify antibodies binding highly similar epitopes.
• Performance Metrics: Reports high precision (0.95) and recall (0.69) for identifying antibodies that bind similar epitopes despite low sequence similarity.
• Comparative and Large-Scale Performance: Demonstrates improved performance relative to general protein-interface tools such as InterComp and provides a speed advantage suitable for immune-repertoire-scale analyses.

Scientific Applications:

• Explore Antibody Diversity: Identify structurally convergent antibodies with divergent sequences to study diverse solutions to the same epitope.
• Enhance Therapeutic Development: Detect alternative antibody candidates that target a desired epitope for therapeutic discovery and optimization.
• Immunological Research: Support immune-repertoire analyses and studies of antibody binding modes to understand immune protection and pathology.

Methodology:

Performs structure-based analysis of antibody-antigen interactions using predicted paratopes from model antibody structures and compares binding modes rather than sequence similarity.