abiview

abiview displays trace data from ABI sequencer files and renders sequencing electropherograms for sequence verification and analysis within the EMBOSS framework.

Key Features:

• Sequencing trace visualization: Renders ABI sequencer file trace data into electropherogram plots that expose nucleotide peak profiles for inspection.
• Integration with EMBOSS: Integrates with the EMBOSS suite and its programming libraries, enabling use alongside other EMBOSS applications and workflows.
• Extensibility and customization: Built on EMBOSS C libraries and supports customization via ACD file definitions and extension of functionality within the EMBOSS framework.

Scientific Applications:

• Sequence verification and quality assessment: Enables verification of base calls and assessment of sequence quality by inspection of ABI trace peak shapes and intensities.
• Mutation and polymorphism validation: Supports detection and validation of mutations or polymorphisms through visual inspection of electropherogram peaks.
• Integration in sequencing workflows: Facilitates incorporation of ABI trace visualization into EMBOSS-based genomic sequencing and analysis pipelines.

Methodology:

Interprets raw ABI sequencing data and renders it as graphical electropherograms using EMBOSS programming libraries.