AcrHub

AcrHub provides an integrated database and analysis platform for anti-CRISPR (Acr) proteins, compiling experimentally validated and predicted Acr sequences to support discovery, comparative analysis, and evolutionary study of Acr-mediated inhibition of CRISPR-Cas systems.

Key Features:

• Comprehensive Database: Catalogs 339 non-redundant experimentally validated anti-CRISPR proteins and over 70,000 predicted Acrs derived from genomic sequence data across diverse prokaryotic organisms and their viruses.
• Predictive Capabilities: Integrates predictive algorithms to identify potential Acr proteins from genomic sequence data.
• Similarity Analysis: Performs sequence and structural similarity comparisons among Acr proteins to aid identification of novel Acrs.
• Phylogenetic Analysis: Infers evolutionary relationships among Acr proteins to explore their origins and diversification.
• Homology Network Analysis: Constructs homology networks linking known and predicted Acrs to assess functional conservation and divergence.

Scientific Applications:

• Gene Editing Research: Supports studies of Acrs to inform control of CRISPR-Cas activity in gene editing applications.
• Phage Therapy Development: Provides data on Acr proteins relevant to bacterial resistance and phage–host interactions for phage therapy research.
• Microbial Evolution Studies: Enables evolutionary analyses of Acr distribution and diversification across prokaryotes and their viruses.

Methodology:

Combines experimentally validated Acr sequences with predictive modeling and applies sequence/structural similarity analysis, phylogenetic analysis, and homology network construction.