ADAPTER REMOVAL

ADAPTER REMOVAL removes adaptor sequences from raw short-read next-generation sequencing (NGS) data to produce cleaned FASTA sequences for small RNA (sRNA) analyses, including microRNA (miRNA) studies.

Key Features:

• Adaptor Fragment Removal: Detects and removes adaptor sequences from raw sequencing reads to prevent artefacts in downstream analyses.
• Output in FASTA Format: Writes cleaned reads in FASTA format for compatibility with downstream bioinformatics tools and databases.
• Scalability and Efficiency: Processes large sRNA datasets generated by NGS within timeframes suitable for high-throughput projects.
• Integration into UEA sRNA Workbench: Integrates with the UEA sRNA Workbench suite to enable downstream sRNA analysis and visualization components.

Scientific Applications:

• RNA silencing research: Provides cleaned sRNA data to support studies of RNA silencing mechanisms and small RNA biology.
• microRNA (miRNA) identification and analysis: Enables accurate identification and characterization of miRNAs from sRNA sequencing datasets.
• sRNA population profiling: Facilitates analysis of sRNA roles in development, pathogen control, genome maintenance, and responses to environmental changes.

Methodology:

Employs algorithms to detect adaptor sequences by aligning potential adaptor sequences within reads and trimming them accordingly, then formats the cleaned reads into FASTA files.