AdipoQ

AdipoQ provides two ImageJ plugins for automated quantitative analysis of adipocyte morphology and function in tissue sections and in vitro cell cultures.

Key Features:

• ImageJ plugin architecture: Two plugins operate within ImageJ to perform automated quantitative analyses on microscopy images.
• Adaptability to imaging modalities: Compatible with histological staining and immunofluorescent labeling across multiple imaging modalities.
• Batch processing: Supports batch processing of large image datasets for high-throughput analysis.
• Comprehensive parameter analysis: Extracts a broad spectrum of morphological and functional parameters, including metrics distinguishing white adipocytes (large unilocular lipid droplets) and brown adipocytes (small multilocular lipid droplets).
• Integration of multiple fluorescent readouts: Analyzes multi-channel fluorescent images to combine several fluorescent markers and readouts.

Scientific Applications:

• Adipose tissue research: Quantifies adipocyte morphology and function to distinguish white and brown adipose tissue and study physiological and pathological changes.
• Clinical assessment: Provides quantitative morphological data on adipocytes that can contribute to analyses relevant to metabolic disorders and adipose tissue dysfunction.

Methodology:

Two ImageJ plugins perform automated, batch-capable analyses on labeled images from cell cultures and ex vivo tissue sections, supporting histological and immunofluorescent staining and multi-channel fluorescence readouts.