AGNOSTOS-DB

AGNOSTOS-DB provides a database of clustered coding sequences and associated functional, ecological, and phylogenetic metadata to unify known and unknown coding sequence spaces.

Key Features:

• Extensive database: Contains high-quality gene clusters enriched with functional, ecological, and phylogenetic information.
• Integration capability: Enables incorporation of new data into existing AGNOSTOS-DB databases to expand and update gene-cluster representation.
• Scalable resource: The seed database comprises 6,572,081 gene clusters linking 342 million genes to support large-scale analyses.
• Diverse data sources: Integrates predicted genes from marine and human metagenomes and from environmental and cultivar genomes across Bacteria, Archaea, Eukarya, and giant viruses.
• Framework component: Serves as the core database component of the AGNOSTOS computational framework that unifies known and unknown coding sequence spaces.

Scientific Applications:

• Functional annotation: Supports generation of functional hypotheses for genes of unknown origin by linking clusters to functional metadata.
• Ecological studies: Enables analysis of gene distribution and ecological context across marine, human, and environmental samples.
• Phylogenetic analysis: Provides phylogenetic metadata to trace evolutionary relationships among gene clusters and organisms.
• Metagenomic research: Facilitates identification and characterization of novel genes from complex microbial communities.

Methodology:

Computational workflows within the AGNOSTOS framework assemble a seed database of 6,572,081 gene clusters linking 342 million predicted genes, integrate predicted genes from marine and human metagenomes and environmental and cultivar genomes, and provide mechanisms to incorporate new data into existing databases.