AGODB

AGODB provides comprehensive domain annotations for argonaute (Ago) proteins to support analysis of their structural and functional domains across eukaryotic (eAgo) and prokaryotic (pAgo) species.

Key Features:

• Comprehensive Coverage: AGODB contains information on 1902 Ago proteins, including both eukaryotic (eAgo) and prokaryotic (pAgo) variants, compiled from research articles and public databases.
• Detailed Domain Annotation: Provides full functional domain annotations for all curated Ago proteins, including detailed annotations of the six domains in long pAgo proteins.
• Gene Ontology (GO) Enrichment Analysis: Integrates GO enrichment analysis results covering biological processes, molecular functions, and cellular components associated with Ago genes across species.
• Curated Provenance: Entries are backed by scientific literature and public database records to document annotation sources.
• Research-oriented Content: Annotations and enrichment results are provided to support studies of RNA-induced silencing complexes in eukaryotes and investigation of pAgo proteins as gene-editing tools.

Scientific Applications:

• Functional Studies: Investigate the roles of specific Ago domains in gene silencing and defense against nucleic acid invaders, including participation in RNA-induced silencing complexes.
• Comparative Genomics: Perform cross-species comparisons of Ago domain architectures to inform evolutionary analyses.
• Gene Editing Research: Support exploration of pAgo proteins as gene-editing tools by providing domain-level annotations relevant to nuclease and binding functions.

Methodology:

Entries were compiled by manual extraction and curation from research articles and public databases, and GO enrichment analysis results were integrated into the dataset.