AhNGE

AhNGE provides RNAseq-based gene expression and isoform information to characterize nodule development in the allotetraploid peanut Arachis hypogaea (Tifrunner) and to support studies of symbiotic nitrogen fixation (SNF).

Key Features:

• RNAseq dataset: Contains RNAseq data from six developmental stages of nodules in the Tifrunner cultivar of Arachis hypogaea.
• Isoform-level expression: Reports expression levels for different splicing variants across nodule development.
• Ortholog analysis: Includes ortholog mappings among four legumes (Cicer, Glycine, Lotus, Medicago) and one non-legume (Arabidopsis) for comparative genomics.
• Allotetraploid context: Focuses on the allotetraploid genome context of Arachis hypogaea for nodule-related gene expression.
• Database integration: Links with PeanutBase to connect Arachis transcriptome data with primary peanut genomic resources.

Scientific Applications:

• Nodule development studies: Characterizing temporal gene expression dynamics across six developmental stages of peanut nodules.
• Comparative genomics: Enabling cross-species comparison of nodule-related genes using orthologs in Cicer, Glycine, Lotus, Medicago, and Arabidopsis.
• Alternative splicing investigation: Assessing the role of splicing variants in nodule development and related molecular processes.
• Symbiotic nitrogen fixation research: Informing molecular investigations of non-canonical features relevant to SNF in peanut.

Methodology:

Processes RNAseq data from six nodule developmental stages of Tifrunner, performs ortholog analysis among Cicer, Glycine, Lotus, Medicago, and Arabidopsis, reports isoform-level expression for splicing variants, and links data with PeanutBase.