All2

All2 performs exhaustive pair-wise comparisons of single-cell and clonal genomes to identify and classify somatic variants—including point mutations, insertions/deletions (indels), and structural variants—distinguishing germline variants, mosaic mutations, and false positives without requiring bulk control samples.

Key Features:

• Pair-wise Comparative Analysis: Performs exhaustive pair-wise comparisons among genomes from different cells or clones to assess variant concordance.
• Versatile Variant Classification: Classifies variant calls (point mutations, insertions/deletions, structural variants) into germline, mosaic, or false positive categories.
• No Bulk Sample Requirement: Operates without bulk control samples, enabling detection of mutations commonly shared across cells.
• Handling Dropped-out Regions: Accounts for regions with dropout events, applicable to whole genome and exome analyses of cloned and amplified cells.
• Enhanced Sensitivity and Accuracy: Improves accuracy and sensitivity of somatic mutation discovery in single cells and multiple clones.

Scientific Applications:

• Reduction of False Positives: Reduces the incidence of false positive variant calls in mutation detection.
• Sensitive Detection of High-Frequency Mutations: Detects high-frequency mutations that may be overlooked by methods relying on bulk comparisons.
• Cell Lineage Tracing: Enables high-resolution cell lineage tracing by accurately filtering and classifying variants.

Methodology:

All2 relies on an analytical framework that performs exhaustive pair-wise comparisons of individual genomes, does not depend on bulk sample data, and considers regions with dropout events to identify and classify point mutations, indels, and structural variants as germline, mosaic, or false positives.