AlPaCas

AlPaCas identifies and analyzes SNV-derived protospacer-adjacent motifs (PAMs) for allele-specific CRISPR gene editing.

Key Features:

• SNV-derived PAM identification: AlPaCas identifies protospacer-adjacent motifs derived from single nucleotide variants in a pathogenic genomic context.
• Cas enzyme selection: AlPaCas provides a list of available Cas enzymes recognizing the SNV-derived PAMs.
• Cas engineering proposals: AlPaCas proposes mutational Cas engineering to enhance selectivity towards SNV-derived PAMs.
• Structural analysis: AlPaCas performs structural analysis of SNV-derived PAMs.

Scientific Applications:

• Allele-specific gene editing: AlPaCas enables precise targeting of disease-causing alleles while preserving wild-type allele functionality for dominant genetic diseases.
• SNV-targeted CRISPR-Cas correction: AlPaCas supports genetic correction of single nucleotide variants, including dominant mutations, through selective disruption or editing of the mutant allele.

Methodology:

AlPaCas uses a sequence-based automated pipeline that accepts gene or SNV input to identify SNV-derived PAMs and analyze their structural properties.