AlphaPulldown

AlphaPulldown performs high-throughput protein–protein interaction screening and structural modeling of protein complexes using AlphaFold-Multimer.

Key Features:

• AlphaFold-Multimer Integration: Uses AlphaFold-Multimer to predict structures of protein complexes and higher-order oligomers.
• Protein–Protein Interaction Screening: Enables systematic screening of candidate protein interactions through large-scale structural modeling.
• Confidence Scoring Metrics: Computes multiple confidence scores to evaluate the reliability of predicted protein–protein interactions and complex structures.
• Structural and Interaction Analysis: Provides computational analysis of predicted complexes and interaction networks.

Scientific Applications:

• Protein–Protein Interaction Discovery: Identifies and evaluates potential protein–protein interactions through structural modeling.
• Structural Biology of Protein Complexes: Predicts structures of multi-protein assemblies and oligomeric complexes.
• Systems Biology Network Analysis: Supports large-scale exploration of interaction networks within cellular systems.

Methodology:

AlphaPulldown performs protein–protein interaction screening by providing protein sequences as input to AlphaFold-Multimer, generating structural models of protein complexes and calculating confidence scores to evaluate predicted interactions.