Alternative Splicing Gallery

Alternative Splicing Gallery visualizes and analyzes alternative splicing events in the human transcriptome by representing transcripts from individual genes as paths within splicing graphs.

Key Features:

• Splicing Graph Representation: Represents transcripts as paths within a splicing graph where bifurcations indicate alternative splicing events among isoforms.
• Integrated Transcript Databases: Aggregates transcript information from Ensembl, RefSeq, STACK, TIGR, and UniGene, including over 1.2 million precomputed putative human transcripts.
• Functional Annotation Integration: Provides access to Gene Ontology (GO) annotations associated with transcript records.
• Sequence Extraction: Enables retrieval of sequences derived from selected exons and neighboring genomic regions.
• Cross-Database Connectivity: Links splicing information to related alternative splicing resources such as ProSplicer.

Scientific Applications:

• Alternative Splicing Analysis: Investigates splice variant diversity and transcript structures across human genes.
• Transcriptome Research: Explores transcript isoform relationships and alternative exon usage within genes.
• Disease and Functional Genomics Studies: Identifies splice variants potentially associated with physiological processes and disease mechanisms.

Methodology:

Alternative Splicing Gallery constructs splicing graphs by integrating transcript data from Ensembl, RefSeq, STACK, TIGR, and UniGene, representing each transcript as a graph path to capture alternative splicing relationships among isoforms.