AMIS

AMIS maps the spatial distribution of G-protein-coupled receptors (GPCRs) in mouse tissues to support functional characterization and drug discovery.

Key Features:

• High-resolution imaging: Generates high-resolution visualizations of GPCR expression patterns across mouse tissues using advanced imaging techniques.
• PRESTO-TANGO and TRUPATH integration: Integrates imaging data with PRESTO-TANGO and TRUPATH assay results to connect receptor localization with functional and ligand-interaction data.
• ZINC virtual library linkage: Links receptors to small-molecule chemistry via the ZINC virtual library, covering billions of small molecules for potential GPCR targeting.
• Structural data incorporation: Incorporates structural data from solved orphan GPCR structures to contextualize expression patterns.
• Genetic model information: Includes genetic information from GPCR knock-in mouse models to relate expression to genotype.
• IDG contribution: Contributes imaging resources to the Illuminating the Druggable Genome (IDG) project for understudied "dark" GPCRs.

Scientific Applications:

• Target identification: Identification and prioritization of novel GPCR therapeutic targets, including understudied "dark" receptors.
• Localization–function correlation: Correlation of receptor localization with specific biological functions and disease states.
• Assay design and interpretation: Design, validation, and interpretation of PRESTO-TANGO and TRUPATH functional assays.
• Structure–genotype contextualization: Integration of imaging with solved GPCR structures and knock-in mouse genetic data to inform structure–function studies.

Methodology:

Generates high-resolution imaging-based visualizations of GPCR expression and integrates these imaging data with PRESTO-TANGO, TRUPATH, the ZINC virtual library, solved orphan GPCR structures, and GPCR knock-in mouse genetic information.