ANARCI

ANARCI annotates and numbers amino acid sequences of antibody and T-cell receptor variable domains to provide standardized residue numbering for sequence comparison and structural analysis.

Key Features:

• Supported Numbering Schemes: Supports five numbering schemes—Kabat, Chothia, Enhanced Chothia, IMGT, and AHo—for annotating residue positions and defining position equivalence.
• Sequence Classification and Numbering: Classifies antibody and T-cell receptor variable-domain sequences and assigns residue numbers according to the chosen numbering scheme.
• Structural-Motif Recognition: Recognizes structural motifs characteristic of variable domains to inform accurate residue annotation.
• Standardized Annotations: Produces standardized residue annotations that enable mapping equivalent positions across different numbering schemes and facilitate downstream analyses.
• Consistency and Accuracy: Provides consistent and accurate numbering across diverse sequence inputs to support comparative and structural studies.

Scientific Applications:

• Sequence Comparison: Enables comparison of antibody and T-cell receptor sequences by providing a common residue-numbering framework.
• Protein Structure Modeling: Supplies standardized residue positions used in protein structure modeling and structural analysis of variable domains.
• Antibody and TCR Engineering: Supports engineering and mutational analyses by precisely locating residues across different numbering schemes.
• Comparative Numbering Analyses: Facilitates studies that require mapping and reconciling residue positions between Kabat, Chothia, Enhanced Chothia, IMGT, and AHo schemes.

Methodology:

ANARCI uses a classification algorithm that recognizes structural motifs in antibody and T-cell receptor variable-domain amino acid sequences and assigns residue numbers according to the selected numbering scheme (Kabat, Chothia, Enhanced Chothia, IMGT, AHo).