AneuFinder

AneuFinder analyzes copy number variations (CNVs) from single-cell whole-genome sequencing (scWGS) to characterize chromosome instability and aneuploidy in cancer.

Key Features:

• Automated CNV Calling: Identifies and annotates copy number changes across individual cells from scWGS data.
• CNV Heterogeneity Quantification: Quantifies heterogeneity of CNVs between cells to assess chromosomal instability within samples.
• Comprehensive Data Analysis: Processes single-cell whole-genome sequencing data to provide an unbiased overview of CNVs at single-cell resolution.
• Visualization and Export: Produces plots of CNV data and exports results for downstream analysis.

Scientific Applications:

• Cancer genomics: Applied to scWGS data to study chromosome instability and aneuploidy in cancers, including lymphomas and leukemias.
• Model and clinical sample analysis: Used to analyze scWGS from p53-deficient mouse models and human B cell leukemias to detect high CNV heterogeneity indicative of ongoing chromosomal instability.

Methodology:

Data processing: handling raw scWGS sequencing data to extract CNV information; annotation and analysis: automatic annotation of copy number changes and quantification of heterogeneity across the cell population; interpretation: generating visualizations and exported results.