AngioQuant

AngioQuant quantifies angiogenesis in in vitro co-culture assays of endothelial cells and fibroblasts by automated image analysis to provide reproducible measurements of tubule structures for evaluation of anti-angiogenic and pro-angiogenic agents.

Key Features:

• Automated Quantification: Performs automated image analysis to measure tubule complexes, including tubule lengths and sizes.
• Junction Analysis: Identifies and counts junctions (branching points) within tubule networks to assess network connectivity.
• Robustness to Illumination Variations: Maintains consistent measurement performance across varying illumination conditions.
• Image Input: Processes images obtained from in vitro angiogenesis assays, including co-cultures of endothelial cells with fibroblasts.

Scientific Applications:

• Cancer research and drug evaluation: Quantitatively evaluates stimulatory and inhibitory effects of candidate anti-angiogenic and pro-angiogenic compounds in endothelial cell–fibroblast co-culture assays.
• Compound-specific analysis: Has been used to demonstrate the inhibitory effect of suramin and the stimulatory effect of vascular endothelial growth factor (VEGF) on angiogenesis.

Methodology:

Processes images from in vitro angiogenesis assays using automated image-analysis algorithms that measure tubule lengths and sizes and identify junctions within networks.