ARCS

ARCS leverages barcoded linked reads from technologies such as 10X Genomics Chromium and GemCode to infer long-range connections and scaffold draft genome assemblies, increasing assembly contiguity.

Key Features:

• Linked-Read Utilization: Uses barcoding information from linked-read technologies (10X Genomics Chromium, GemCode) to recover long-range linkage information absent from conventional short-read data.
• Enhanced Contiguity: Increases assembly contiguity—for example, using moderate coverage (25-fold) Chromium data ARCS improved an ABySS human genome assembly contiguity by over six-fold.
• Scalability and Utility: Integrates barcode-derived linkages to connect high-quality sequences within draft assemblies across diverse genomic projects.

Scientific Applications:

• Genome Assembly: Scaffolds fragmented draft genomes into more contiguous sequences that better reflect chromosomal architecture.
• Structural Genomics: Provides long-range linkage information to aid resolution of complex genomic regions and structural variants.
• Comparative Genomics: Produces higher-quality assemblies that enable more accurate comparative and evolutionary analyses.

Methodology:

Accepts linked-read sequencing data, extracts and interprets barcode information to establish linkage relationships across contigs, and uses those inferred linkages to scaffold draft assemblies and increase contiguity.