ARGs-OSP

ARGs-OSP maps the distribution and mobility of antibiotic resistance genes (ARGs) across bacterial genomes, plasmids, integrons, and metagenomes to support comparative ecological and clinical analyses.

Key Features:

• Extensive Database: Profiles are derived from a dataset comprising 55,000 bacterial genomes, 16,000 plasmid sequences, 3,000 integron sequences, and 850 metagenomes for comparative ARG analyses.
• Standardized Analysis Pipeline: Employs a standardized pipeline to ensure consistent and reliable cross-sample comparisons.
• Focus on Mobile ARGs: Identifies mobile ARGs and reports that over 80% of known ARGs are not associated with plasmids or integrons, with examples including tetracycline and beta-lactam resistance genes such as tetA, tetM, and class A beta-lactamase.
• Integration with Global Data: Integrates data from human-associated and non-human-associated habitats to characterize environmental reservoirs of ARGs.
• Correlation Analysis: Performs correlation analyses between ARG abundance and class 1 integrases (intI1), revealing poor linear correlation across habitats and no significant correlation with anthropogenic factors.

Scientific Applications:

• Risk Assessment: Provides detailed ARG distribution profiles to inform environmental and public health risk assessments of antibiotic resistance.
• Evolutionary Studies: Enables investigation of the evolutionary dynamics and dissemination of ARGs across bacterial taxa and mobile genetic elements.
• Clinical Relevance: Identifies clinically relevant mobile ARGs to support understanding of resistance mechanisms in pathogens.

Methodology:

Metagenomic analysis combined with a standardized analysis pipeline to generate consistent ARG distribution profiles from genomic, plasmid, integron, and metagenomic datasets.