ASEB

ASEB predicts lysine acetylation sites and assigns the responsible KAT-family lysine acetyltransferases using set enrichment analysis to link acetyltransferase activities to substrate lysines.

Key Features:

• Prediction of KAT-specific acetylation sites: Predicts which KAT-families are responsible for acetylating specific lysine residues or proteins.
• Set enrichment analysis: Applies Acetylation Set Enrichment Based set enrichment analysis to associate acetylated sites with KAT-family signatures.
• Integration of mass spectrometry data: Incorporates large-scale mass spectrometry-derived acetylation datasets to inform predictions of modified lysines.
• Integration with protein-protein interaction networks: Uses protein-protein interaction data to provide interaction context and enhance predictive accuracy.

Scientific Applications:

• Gene expression regulation: Supports investigation of how lysine acetylation affects transcriptional regulation via predicted KAT–substrate relationships.
• Protein stability analysis: Aids studies of acetylation impacts on protein stability by identifying likely acetylated lysines and their modifying KAT-families.
• Metabolism regulation studies: Facilitates exploration of lysine acetylation roles in metabolic pathway regulation through site- and KAT-specific predictions.

Methodology:

Applies set enrichment analysis to large-scale mass spectrometry-derived acetylation datasets combined with protein-protein interaction networks to predict KAT-family-specific lysine acetylation.