ASHLEYS

ASHLEYS automates quality control of single-cell DNA template strand sequencing (Strand-seq) libraries to identify high-quality libraries for downstream haplotype phasing, phased assembly construction, sister-chromatid exchange mapping, and structural variant discovery.

Key Features:

• Automation: Automates the initial quality control phase for single-cell Strand-seq libraries to reduce manual curation.
• Performance: Delivers near-expert accuracy in identifying high-quality libraries for downstream genomic analyses.
• Speed: Labels extensive single-cell Strand-seq datasets in seconds to accelerate dataset selection.

Scientific Applications:

• Chromosome-length haplotype phasing: Aids accurate phasing of haplotypes across entire chromosomes using selected high-quality Strand-seq libraries.
• Phased assemblies construction: Supports generation of phased genome assemblies by providing reliably phased input libraries.
• Mapping sister-chromatid exchange events: Enables identification and mapping of sister-chromatid exchange events from Strand-seq data.
• Structural variant discovery: Facilitates discovery of structural variants by ensuring high-quality Strand-seq libraries are used for analysis.

Methodology:

Employs advanced algorithms, including machine learning techniques and domain-specific heuristics, to evaluate quality metrics of single-cell Strand-seq libraries.