assayM

assayM monitors mutations in the SARS-CoV-2 genome that affect RT-PCR primer and probe complementarity to assess impacts on diagnostic assay binding and accuracy.

Key Features:

• Mutation Monitoring: Identifies mutations within RT-PCR primer and probe target regions, including World Health Organization (WHO)-published and user-supplied primer/probe sequences.
• Global Dataset Integration: Uses globally available SARS-CoV-2 genomic datasets to quantify and contextualize mutation prevalence and distribution across sequences.

Scientific Applications:

• Diagnostic Accuracy Maintenance: Tracks mutations that may disrupt primer/probe binding to inform updates to RT-PCR assays and reduce the risk of false negatives or positives.
• Viral Evolution Studies: Enables analysis of SARS-CoV-2 mutation patterns across global genomes to support epidemiological and virological research.

Methodology:

Compares primer and probe sequences against comprehensive global SARS-CoV-2 genome datasets to detect and monitor genetic variations that could impact diagnostic assay binding.