AssemblX

AssemblX assembles multigene DNA constructs to enable construction of complex genetic circuits and biological pathways in synthetic biology and biotechnology.

Key Features:

• Scalability: Supports assembly of up to 25 functional units from over 75 subunits, including promoters, coding sequences, and terminators.
• Overlap-Based Cloning: Employs overlap-based, scar-free cloning methods that are sequence-independent at the initial assembly level.
• Rare Cutting Homing Endonucleases: Utilizes rare cutting homing endonucleases for standardized, polymerase chain reaction-free assembly into multigene modules, with computationally optimized overlap sequences to ensure precise and efficient gene integration.
• Marker Switching Strategies: Incorporates selection and marker switching strategies to enable transfer of assembled constructs to desired expression hosts.

Scientific Applications:

• Genetic Circuit Engineering: Construction of large genetic circuits composed of multiple genes and regulatory parts in synthetic biology.
• Multigene Pathway Assembly: Assembly of entire biological pathways for metabolic engineering and biotechnology applications.

Methodology:

Computationally optimized overlap sequence design using computational algorithms for overlap design.