assemblyManager

assemblyManager assembles synthetic DNA constructs and generates robotic commands to enable high-throughput construction of composite parts for synthetic biology applications.

Key Features:

• Automation and Scalability: Supports automated, scalable assembly workflows for constructing extensive libraries of synthetic DNA.
• Hardware Integration: Integrates with a minimal hardware setup including a Biomek 3000 liquid handling robot, a benchtop centrifuge, and a plate thermocycler to execute assembly protocols.
• High-Throughput Capability: Enables parallel processing to assemble several hundred large DNA constructs with high fidelity and a reported success rate exceeding 96%.
• Iterative 2ab Reaction Protocols: Supports iterative 2ab reactions for assembly of BglBrick standard biological parts.
• Robotic Command Computation: Accepts lists of composite parts described by basic part composition and computes the robotic commands required for 2ab assembly execution.

Scientific Applications:

• Synthetic Biology Research: Facilitates rapid construction of standardized genetic components for synthetic biology experiments.
• Genomic Engineering: Supports concurrent assembly and testing of multiple DNA constructs for genomic engineering applications.
• Biotechnology Development: Enables efficient creation of custom DNA sequences for biotechnological research and product development.

Methodology:

Takes a list of composite parts described in terms of their basic part composition and computes the robotic commands required for executing 2ab assembly reactions on a Biomek 3000 platform, providing control over liquid handling and thermal cycling steps.