athisomiRDB

athisomiRDB houses 20,764 isomiRs derived from Arabidopsis thaliana miRNAs, enabling exploration of isomiR presence or absence across >2,700 diverse small RNA-sequencing samples at the sample, miRNA, or isomiR levels to support research into the regulatory nature of the Arabidopsis miRNAome.

Key Features:

• IsomiR Classification: The database categorizes isomiRs as exclusive or ambiguous, with annotations for transcriptional origin and variant-containing isomiRs.
• Genomic Variant Integration: IsomiRs include identifiers for frequent single-nucleotide polymorphisms from the 1001 Genomes Project.
• Post-Transcriptional Modification Data: The database provides 3' nontemplated post-transcriptional additions for isomiRs.
• Functional Annotations: IsomiR-target interactions and trait associations are provided for each isomiR.
• Multi-Level Exploration: Users can explore data at the sample, miRNA, or isomiR levels with information on isomiR presence or absence.

Scientific Applications:

• IsomiR Biology Research: The database supports investigation of isomiRs resulting from imprecise cleavage during pre-miRNA processing and post-transcriptional alterations in plants.
• miRNAome Regulation Studies: athisomiRDB facilitates unraveling the regulatory nature of the Arabidopsis miRNAome through extensive sRNA-seq datasets.
• Target Regulation Analysis: The database enables study of isomiRs as crucial regulators of target microRNAs in Arabidopsis.
• Trait Association Research: Trait associations provided for each isomiR support research linking isomiR variation to phenotypic outcomes.

Methodology:

IsomiRs were identified and annotated from Arabidopsis small RNA-sequencing libraries comprising >2,700 diverse samples.