auto-DRRAFTER

auto-DRRAFTER reconstructs RNA coordinates within moderate-resolution cryogenic electron microscopy (cryo-EM) density maps using Rosetta-based modeling to enable structural interpretation of RNAs and ribonucleoprotein (RNP) complexes.

Key Features:

• Rosetta integration: Uses Rosetta modeling algorithms to place and refine RNA coordinates within cryo-EM density maps.
• Automated workflow: Implements an automated, stepwise protocol for RNA model building exemplified on a glycine riboswitch from Fusobacterium nucleatum.
• Moderate-resolution support: Targets modeling challenges specific to moderate-resolution cryo-EM maps to recover RNA coordinates.
• Application to RNAs and RNPs: Supports modeling of standalone RNAs and RNA components within ribonucleoprotein complexes.

Scientific Applications:

• RNA structural modeling: Constructing RNA models that align with experimental cryo-EM density for downstream structural analysis.
• RNP complex interpretation: Placing RNA coordinates within RNP complexes to inform studies of RNA–protein interactions.
• Structure–function analysis: Facilitating interpretation of RNA conformations to support hypotheses about biological roles.

Methodology:

Integrates Rosetta-based modeling algorithms with cryo-EM density maps to trace and build RNA coordinates and implements an automated, stepwise protocol exemplified by modeling a Fusobacterium nucleatum glycine riboswitch.