AutoScanJ

AutoScanJ automates image-based target detection and microscope control to enable targeted high-resolution imaging in motorized fluorescence microscopy of live and fixed samples.

Key Features:

• Automated Target Detection: Automatically identifies targets of interest in images and directs a motorized microscope to their precise locations for imaging.
• High-Resolution Imaging for Live Samples: Detects biological events at onset and acquires high-resolution images of transient processes in live specimens.
• Data Acquisition Optimization for Fixed Samples: Reduces data volume and acquisition time by focusing imaging on statistically significant targets within each field of view.
• Compatibility with Microscope Control Platforms: Interfaces with motorized fluorescence microscopes via Leica LAS AF/X and Micro-Manager.
• Customization and Extensibility: Provides target detection functions and ImageJ macro-compatible scripts for developing and sharing custom image analysis workflows.

Scientific Applications:

• Micropatterned-surface imaging: Enables targeted imaging of samples fixed on micropatterned surfaces.
• Live cell division monitoring: Enables detection and high-resolution imaging of live cells undergoing multiple rounds of division.

Methodology:

Implemented as a suite of ImageJ scripts and macro functions that perform automated target detection and control motorized microscopes via Leica LAS AF/X or Micro-Manager.