BactEXTRACT

BactEXTRACT converts raw microtitre plate reader optical density, fluorescence, and luminescence measurements into analyses and publication-ready visualizations of bacterial growth and gene expression.


Key Features:

  • Data types supported: Processes raw optical density, fluorescence, and luminescence measurements acquired from microtitre plate readers.
  • Growth-curve and expression analysis: Derives bacterial growth curves and gene expression profiles from processed measurement data.
  • Visualization and export: Produces publication-ready plots directly from processed datasets.

Scientific Applications:

  • Microbial physiology: Quantifies growth kinetics and population behavior from optical density measurements.
  • Genetics: Assesses gene expression changes using fluorescence and luminescence reporter measurements.
  • Systems biology: Integrates growth and expression measurements to support comparative and systems-level analyses across strains or conditions.

Methodology:

Converts and processes raw optical density, fluorescence, and luminescence measurements from microtitre plate readers into processed datasets and publication-ready plots.

Topics

Details

Cost:
Free of charge
Tool Type:
web application
Operating Systems:
Mac, Linux, Windows
Programming Languages:
R
Added:
4/19/2024
Last Updated:
11/24/2024

Operations

Publications

Dénéréaz J, Veening J. BactEXTRACT: an R Shiny app to quickly extract, plot and analyse bacterial growth and gene expression data. Access Microbiology. 2024;6(1). doi:10.1099/acmi.0.000742.v3. PMID:38361656. PMCID:PMC10866030.

PMID: 38361656
Funding: - European Research Council: 771534-PneumoCaTChER - Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung: 310030_192517, 310030_200792, 51NF40_180541

Links