BALROG-ISO

BALROG-ISO automates the analysis of bacterial isolate whole genome sequences through a Nextflow pipeline that performs taxonomic classification, genomic annotation, antimicrobial resistance gene annotation, and prediction of ARG origin.

Key Features:

• Taxonomic Classification: Performs taxonomic classification of bacterial isolate whole genome sequences.
• Genomic Annotation: Provides genomic annotation as part of the automated analysis workflow.
• Antimicrobial Resistance Gene Annotation: Annotates antimicrobial resistance genes (ARGs) in bacterial genomes.
• ARG Origin Prediction: Predicts the origin of ARGs, such as plasmid or chromosomal.
• Summary Report Generation: Generates a comprehensive summary report visualizing key quality metrics and annotation results.
• High-Throughput Processing: Enables concurrent analysis of multiple genomic samples through streamlined modular processes.

Scientific Applications:

• Antimicrobial Resistance Surveillance: Supports One Health approaches to exploring antimicrobial resistance in bacterial genomes.
• Bacterial Genome Characterization: Facilitates comprehensive characterization of bacterial isolate genomes including resistance determinants and their genomic context.

Methodology:

BALROG-ISO is implemented as a Nextflow pipeline with modular processes that minimize command inputs and reduce manual job submission.