BAR-Seq

BAR-Seq enables clonal tracking of gene-edited cells by analyzing barcode sequencing data derived from homology-directed targeted integration of barcoding cassettes.

Key Features:

• Clonal Tracking Capability: Enables analysis of clonal dynamics of cells edited with engineered nucleases through barcode sequencing.
• Broad Applicability: Applicable across diverse gene editing strategies for analysis of engineered clones in basic and translational research.
• Efficient Workflow: Supports rapid library preparation and validation followed by clonal analyses that can be completed in approximately one week.

Scientific Applications:

• Hematopoietic Stem/Progenitor Cell Clonal Analysis: Investigates clonal dynamics of human edited hematopoietic stem/progenitor cells in xenotransplanted hosts.
• Comparative Evaluation of Editing Protocols: Compares gene editing protocols at the clonal level to inform optimization of editing strategies.

Methodology:

Performs clonal tracking analyses on raw barcode sequencing data generated from homology-directed targeted integration of barcoding cassettes.