bbeaR

bbeaR facilitates epitope-specific antibody profiling from Bead-Based Epitope Assay (BBEA) data to analyze immunoglobulin repertoires (IgE, IgG, IgA, IgD) in studies of inflammatory disorders such as allergies.

Key Features:

• Multiplex Immunoassay Integration: Tailored for the Bead-Based Epitope Assay (BBEA) to quantify IgE, IgG, IgA, and IgD specific to distinct epitopes.
• Data Processing Capabilities: Imports, processes, and normalizes .csv files exported from the Luminex reader for downstream analysis.
• Quality Control Evaluation: Evaluates quality-control metrics to assess reliability and accuracy of assay results.
• Differential Epitope-Binding Analysis: Supports linear modeling to identify differential epitope-binding antibodies across conditions or populations.
• Visualization Tools: Generates visualizations to present analysis results and facilitate interpretation.
• Epitope Mapping: Maps epitope amino-acid sequences onto corresponding primary protein structures.

Scientific Applications:

• Allergy research: Enables detailed profiling of epitope-specific antibodies to investigate mechanisms underlying allergic disease.
• Inflammatory disorder studies: Profiles epitope-specific immunoglobulin repertoires in research on inflammatory disorders.
• Comparative isotype analysis: Facilitates analysis of differential binding patterns across immunoglobulin isotypes.

Methodology:

Integrates BBEA data by importing, processing, and normalizing Luminex .csv output, evaluating quality-control metrics, applying linear modeling and other statistical and visualization techniques within the R environment, and mapping epitope amino-acid sequences onto primary protein structures.