BC-store

BC-store analyzes and selects barcode sets for multiplexed sequencing on MGI Tech platforms to optimize barcode compatibility and nucleotide diversity across sequencing cycles.

Key Features:

• Compatibility Analysis: Assesses compatibility of MGI Tech barcode sets within a flow cell lane for equal and arbitrary (including non-equimolar) sample fractions.
• Barcode Management: Allows adding new barcodes to existing sets with configurable sharing options.
• Efficiency Optimization: Optimizes grouping of barcodes to maximize nucleotide diversity across sequencing cycles and avoid identical adapter nucleotides in the same cycle.
• Lane Distribution Recommendations: Generates recommendations for optimal sample distribution across flow cell lanes to improve sequencing quality.
• Implementation: Implemented in Python3.

Scientific Applications:

• Sample Multiplexing: Supports design and evaluation of barcode sets for multiplexed NGS runs on MGI Tech platforms.
• Non‑equimolar Pooling: Enables planning and assessment of pooled libraries with arbitrary sample fractions, including non‑equimolar pools.
• High‑Throughput Sequencing Studies: Applicable to genomics, transcriptomics, and metagenomics projects that require precise barcode management for high-throughput sequencing.

Methodology:

Users input MGI Tech barcode sets which BC-store analyzes for compatibility and efficiency by evaluating nucleotide diversity across sequencing cycles and producing recommendations for sample distribution across flow cell lanes.