BEHAV3D

BEHAV3D combines multi-color live 3D imaging with computational analysis to study tumor-immune interactions and assess how solid and liquid tumors respond to cellular immunotherapy.

Key Features:

• Tumor death dynamics analysis: Quantitative analysis of tumor cell death dynamics at single-organoid, single-cell, and population levels.
• T cell behavior classification: Classification of T cell behaviors involved in tumor targeting.
• Data-informed 3D visualization: Generation of 3D visualizations and videos for detailed inspection and interpretation of results.
• Heterogeneity capture: Captures both inter- and intratumoral heterogeneity in treatment outcomes.

Scientific Applications:

• Immunotherapy response assessment: Refined assessment of how solid and liquid tumors respond to cellular immunotherapy.
• T cell mode of action studies: Reveals behavioral patterns and mechanistic insights into T cell tumor targeting.
• Immunotherapy product comparison: Enables comparing, prioritizing, and improving immunotherapy products by highlighting behavioral differences between individual tumor donors, distinct T cell therapy concepts, or subpopulations.

Methodology:

Multi-color live 3D imaging with live cell dyes, segmentation-based image processing to track individual organoids, tumor and immune cells, and an analytical pipeline for behavioral profiling.