BEScreen

BEScreen provides a comprehensive toolkit for designing base editing libraries with variant-, gene-, and region-centric modes to enable high-throughput screening of genetic variants for phenotypic effects.

Key Features:

• Variant-centric mode: Accepts genomic coordinates, amino acid changes, or rsIDs as input for guide design.
• Gene-centric mode: Designs near-saturation libraries covering the entire coding sequence of given genes or transcripts.
• Region-centric mode: Designs all possible guides for given genomic regions.
• Biological consequence filtering: Enables selection of guides by biological consequence.
• Base editor customization: Features comprehensive customization of base editor characteristics.
• Variant annotation: Offers optional annotation using Ensembl's Variant Effect Predictor.

Scientific Applications:

• Base editing screen design: Supports design of single guide RNA libraries for gene- or variant-centric base editing screens.
• High-throughput variant screening: Enables screening of genetic variants for phenotypic effects at scales from individual variants to large, near-saturation libraries.

Methodology:

BEScreen is provided as a web server and command-line tool.