BFPtool
BFPtool analyzes videomicroscopy recordings from Biomembrane Force Probe (BFP) experiments to support single-molecule force spectroscopy and studies of biological interfaces.
Key Features:
- Tailored image processing: Applies advanced image processing capabilities specifically tailored for BFP experiment videomicroscopy recordings.
- Pipette tracking: Implements pipette tracking to determine pipette position within video recordings.
- Unstable/out-of-focus handling: Processes recordings from repeated trials that may be unstable or lose focus.
- BFP force range support: Supports analysis of BFP experiments operating in a force range of 0.1 pN to 1000 pN.
Scientific Applications:
- Single-molecule force spectroscopy: Analyze BFP videomicroscopy data to study single-molecule force interactions.
- Biological interface studies: Analyze interactions at biological interfaces using BFP videomicroscopy recordings.
Methodology:
Applies advanced image processing to videomicroscopy recordings from Biomembrane Force Probe experiments and performs pipette tracking.
Topics
Details
- License:
- GPL-3.0
- Tool Type:
- plugin
- Operating Systems:
- Linux, Windows, Mac
- Programming Languages:
- MATLAB
- Added:
- 7/19/2018
- Last Updated:
- 12/10/2018
Operations
Data Inputs & Outputs
Transmembrane protein analysis
Outputs
Publications
Šmít D, Fouquet C, Doulazmi M, Pincet F, Trembleau A, Zapotocky M. BFPTool: a software tool for analysis of Biomembrane Force Probe experiments. BMC Biophysics. 2017;10(1). doi:10.1186/s13628-016-0033-2. PMID:28289540. PMCID:PMC5304404.
PMID: 28289540
PMCID: PMC5304404
Funding: - Grantová Agentura Ceské Republiky: 14-16755S
- Ministerstvo Školství, Mládeže a Telovýchovy: 7AMB12FR002
- Grantová Agentura, Univerzita Karlova: 396213
- Agence Nationale de la Recherche: ANR-2010-BLAN-1401-01
- National Institutes of Health: 5R01DC012441