BindCraft

BindCraft leverages AlphaFold2 weights to generate de novo protein binders with nanomolar affinity without requiring high-throughput screening or experimental optimization.

Key Features:

• AlphaFold2-based design: Uses AlphaFold2 weights to predict and generate protein-protein interactions.
• Automated pipeline: Provides an open-source, automated workflow for binder design.
• High success rates: Achieves experimental success rates of 10-100% for functional binder design.
• Target flexibility: Designs binders even in the absence of known binding sites.

Scientific Applications:

• Allergen inhibition: Reduces IgE binding to birch allergen in patient-derived samples.
• Gene editing modulation: Modulates CRISPR-Cas9 gene editing activity through designed binders.
• Toxin neutralization: Reduces cytotoxicity of foodborne bacterial enterotoxins.
• Targeted gene delivery: Redirects adeno-associated virus capsids using cell-surface-receptor-specific binders for targeted gene delivery.
• Challenging target design: Generates binders against cell-surface receptors, common allergens, de novo designed proteins, and multi-domain nucleases.

Methodology:

BindCraft uses AlphaFold2 weights for computational prediction and generation of protein binders.