barbieQ

barbieQ provides robust statistical tools for analyzing barcode count data from cell clonal tracking (lineage tracing) experiments to quantify clone population abundance under experimental perturbations.

Key Features:

• Barcode Count Data Preprocessing: Prepares raw barcode count data for downstream statistical analysis.
• Statistical Testing: Performs robust statistical tests on barcode count data to assess clone behavior differences.
• Visualization: Generates visual representations of clonal tracking and barcode count analysis results.

Scientific Applications:

• Lineage Tracing Analysis: Tracks cell clone behaviors and population dynamics through inherited DNA barcode sequences.
• Clone Abundance Quantification: Measures population abundance of individual clones under specific experimental perturbations by counting barcodes.

Methodology:

The package analyzes barcode count data based on the one-to-one mapping of unique DNA barcode sequences to cell clones, where barcodes incorporated into the initial cell's DNA are inherited within the lineage.