crisprBowtie

crisprBowtie maps on-targets and off-targets of CRISPR gRNA spacer sequences using bowtie alignment.

Key Features:

• Fast alignment: Performs bowtie-based alignment of gRNA spacer sequences.
• Custom nuclease support: Alignments can be performed using either commonly-used or custom CRISPR nucleases.
• Flexible genome compatibility: Alignment works with any reference or custom genomes.
• Nuclease type support: Both DNA- and RNA-targeting nucleases are supported.

Scientific Applications:

• On-target mapping: Identifies intended target sites for CRISPR gRNA spacer sequences.
• Off-target mapping: Identifies potential unintended target sites for CRISPR gRNA spacer sequences.

Methodology:

Uses bowtie for sequence alignment of CRISPR gRNA spacer sequences against reference or custom genomes.