crisprBwa

crisprBwa maps on-targets and off-targets of CRISPR gRNA spacer sequences using BWA alignment.

Key Features:

• Fast BWA alignment: Performs rapid alignment of CRISPR gRNA spacer sequences.
• Flexible nuclease support: Can be performed using either commonly-used or custom CRISPR nucleases.
• Custom genome compatibility: Alignment works with any reference or custom genomes.

Scientific Applications:

• CRISPR gRNA target mapping: Identifies on-target and off-target sites for CRISPR gRNA spacer sequences.

Methodology:

Uses BWA for sequence alignment of CRISPR gRNA spacer sequences against reference or custom genomes.