CytoPipelineGUI

CytoPipelineGUI provides interactive Shiny-based graphical interfaces for visualizing and assessing flow cytometry data analysis pipelines constructed with CytoPipeline.

Key Features:

• Interactive Flow Frame Assessment and Comparison: Enables interactive evaluation and comparison of flow cytometry data frames within pre-processing pipelines.
• Interactive Scale Transformations Visualization and Adjustment: Supports visual inspection and modification of scale transformations applied to flow cytometry data.

Scientific Applications:

• Pre-processing Pipeline Visualization: Facilitates visual assessment of automated pre-processing pipelines for flow cytometry data, including compensation, unmixing, scale transformation, debris removal, doublet removal, dead cell removal, and batch effect correction.
• Pipeline Benchmarking Support: Provides visualization utilities to interpret benchmark metrics when comparing pre-processing pipelines differing by their quality control methods.

Methodology:

CytoPipelineGUI implements two Shiny applications as companion visualization tools to the CytoPipeline R package.