flowGate

flowGate enables manual GUI-based gating of flow cytometry data in R through an interactive Shiny application, allowing users to define cell populations by drawing gates directly on data plots rather than specifying coordinates.

Key Features:

• Interactive Gate Drawing: Users draw 1D and 2D span/rectangle gates, quadrant gates, and polygon gates directly on plots of flow cytometry data.
• Coordinate-Free Gating: Gate boundaries are defined through interactive graphical selection rather than manual coordinate entry.

Scientific Applications:

• Flow Cytometry Analysis: Manual gating of flow cytometry data to define and isolate cell populations for downstream analysis in R.

Methodology:

The tool implements interactive gating through a Shiny application interface, integrating with R-based flow cytometry workflows.