flowSpecs

flowSpecs processes high-dimensional spectral cytometry data through spectral decomposition, transformation, visualization, and cleanup to enable downstream analyses.

Key Features:

• Spectral Decomposition: Performs spectral decomposition on spectral cytometry data.
• Data Transformation: Transforms flowFrames and flowSets to dataframes to aid downstream analyses such as with DepecheR.
• Visualization: Provides visualization capabilities for cytometry data.
• Data Cleanup: Implements cleanup procedures for pre-processing cytometry data.
• Automatic 1D-Gating/Filtering: Functions for flowCore-compliant automatic 1D-gating/filtering are in development.

Scientific Applications:

• High-Dimensional Cytometry Pre-processing: Fills the role of conventional cytometry pre-processing software for spectral cytometry data.
• Downstream Analysis Integration: Enables transformation of flowCore objects to dataframes for compatibility with downstream analysis tools such as DepecheR.

Methodology:

The package performs spectral decomposition, transformation, visualization, and cleanup on flowFrames and flowSets, with flowCore-compliant automatic 1D-gating/filtering functions in development.