metagene2

metagene2 produces metagene plots to compare coverages of sequencing experiments at selected groups of genomic regions.

Key Features:

• Coverage normalization: Normalizes coverages as part of the analysis pipeline.
• Plot facetting: Facets plots according to experimental metadata.
• Bootstrap confidence intervals: Uses bootstraping analysis to provide confidence intervals of per-sample mean coverages.

Scientific Applications:

• Protein binding assessment: Assesses the binding of DNA-interacting proteins at promoter regions.
• Antisense transcription survey: Surveys antisense transcription over the length of a gene.

Methodology:

Bootstraping analysis is used to provide confidence intervals of per-sample mean coverages.